Always use two hands when moving the microscope. Ensure the microscope is not plugged in before moving it. If usingimmersion oil, be sure toclean the immersion oil lensthoroughly after use. Keeping your microscope clean(especially the optics) will also ensure that it lasts longer. ...
(e.g. Mowiol or Prolong Gold) is used which fixes the sample on a microscope slide and which also prevents its dehydration. In addition, mounting media increase the refractive index, which is conducive for microscopy with oil immersion objectives. Several manufacturers offer mounting media with ...
Z-series images were obtained through the ENCC network at 2.5-, 3-, 4- or 5-minute intervals using X10, X20 or X40 oil immersion objectives. Cell tracking (manual and automatic), and measurements of angles and distances, were performed on videos of projected z-series using Axiovision (...
A modern compound microscope often has two eyepieces, or oculars, with one lens each so that you can have binocular vision. A prism at the juncture of the oculars with the body splits the image for each side. There are generally three to four objective lenses at the other end of the ...
DiD-labeled DENV (opsonized and non-opsonized) was added in situ to P388D1 cells in the presence or absence of the indicated inhibitors. After 30 min of infection at 37 °C, the cells were washed and snapshots were taken with an oil-immersion 100× objective. (A–D) Representative...
The spinal cords were cut into 30 μm cryosections using Hyrax C60 cryostat (Carl Zeiss) and mounted onto Superfrost Plus microscope slides (Thermo Fisher Scientific). Spinal cord section were incubated at 4°C overnight in a primary antibody solution (PBS, 0.3% Triton X-100, 10% normal ...
Mycoplasma and viruses infect cells and, due to their size, are difficult to detect directly. Eukaryotic contamination often consists of fast-growing cells that are visible under a microscope, but can be hard to distinguish from the cultured cells and can gradually take over a culture [1]. ...
It is also mixed with edible oil, chilies, and salt (pickled) and preserved in closed containers for up to two years. Like khorisa, Lactobacilli are largely active in the poka khorisha fermentation stage. The fermented tabah bamboo shoot pickle is one of the means to enhance the shelf-...
The instrument utilized a Nikon (Melville, NY) TE2000U inverted TIRF microscope; a Nikon Apochromat 60×, NA 1.49 TIRF oil immersion objective; and a CNI-Laser 300 mW, 532 nm, diode-pumped solid-state laser model MGLIII-532-300 mW. The laser power exiting the objective was reduced by ...
(ROI) function on the confocal microscope.Z-series images were obtained through the ENCC network at 2.5-, 3-, 4- or 5-minute intervals using X10, X20 or X40 oil immersion objectives. Cell tracking (manual and automatic), and measurements of angles and distances, were performed on videos ...