If you plot enzyme velocity as a function of subtrate concentration, you can fit the data to the Michaelis-Menten equation to determine the Kmand Vmax. The Vmaxis the maximum enzyme velocity extrapolated out to very high concentrations of substrate. It is expressed in the same units you used...
Press Enter and drag down the Fill Handle to see the results. Read More: How to Plot Sieve Analysis Graph in Excel STEP 6 – Find the Sum of Squares of the Differences To find the best values for Km and V-max, we need to determine the sum of squares of the differences. Select Cell...
A matrix organizes values into rows and columns for them to be used in solving various mathematical problems. A number of concepts have been applied in the calculation of eigenvectors. One method used includes equations that solve the matrices....
If the enzyme molecule has more than one active site, then we multiply [E]t by the number of active sites to determine its effective concentration. Determine the value of the turnover number of the enAt what substrate concentration would an enzyme with a Km of 0.00...