Therefore, we were not able to determine if there is a gene dependency for the best method. To resolve this matter, a wider range of expression levels per gene should be examined. We also tested if the correlations between the microarray and Real Time RT-PCR data depended on signal ...
1. DNA 2. RNA 3. Both RNA and DNA 4. Neither RNA nor DNA. Why is RNA not a genetic material? How can you determine the sequence of DNA, RNA, and protein? How did the first RNA molecule form when it was continuously bombarded by millions of other atoms?
How are DNA and mRNA alike? How does DNA structure relate to its function? How are proteins and nucleic acids related? What are some similarities between transcription and DNA replication? How does DNA determine the traits of an organism?
To determine chemically similar amino acids, we used an amino acid similarity matrix based on binding covariance of different short peptides to MHC (Major Histocompatibility Complex)42. As noted in the original study42, we identified chemically similar amino acids from pairs of amino acids whose ...
not tissue- or cell-specific, this approach may not be sufficient to determine the specific tissue in which the expression is detected [7]. To overcome these challenges, the ideal solution is the isolation of specific tissues. In the case of secondary growth, it is possible to manually ...
to determine the presence or expression in body fluid cells of at least one cancer-specific gene that is not expressed in non-cancer cells or of at least one cancer-associated gene, and of at least one cancer-specific or cancer-associated gene in cancer cells removed from the body fluid ce...
Typical RT-PCR conditions include the use of up to 5µg of total RNA or up to 100ng of polyA+ mRNA, 20–30 units of enzyme and a 60-minute incubation at 42°C. AMV RT is more processive than M-MLV RT (5–6), so fewer units are required to generate the same amount of cDNA...
lung cancer patients2. These findings will help determine which patients should be treated with immunotherapy alone and which will require additional chemotherapy3. “Being able totailor therapeutic interventions based on blood-borne microRNAs could lead to marked improvements in patient outcomes,” he ...
Describe how nucleosomes function to condense DNA. How can DNA be transcribed to RNA? How is DNA replicated? How is mRNA synthesized from DNA? How does DNA replicate? How do you replicate a strand of DNA? How is DNA organized in the nucleus in dividing and nondividing cells?
The array of sequence variants was used to determine the patient's haplogroup. The haplogroup-associated variants were identified and the population frequency of each nucleotide sequence variant was calculated relative to the MITOMASTER and Pereira et al26 databases. To identify rare variants, we ...