Prism can easily fit a dose response curve to determine the IC50. From the Welcome dialog, choose the XY tab, drop the list of sample data sets and choose "RIA or ELISA". Note that the X values are logarithms of concentration. Prism offers built-in equations designed to handle X values ...
Nonlinear regression can determine a drug’s potency (EC50 or IC50), find best-fit values, compare results from different experiments and interpolate unknowns from a standard curve. For example, you can study the effect of treating cells with an agonist in the presence and absence of an antago...
To understand why a particular sensor code has been set if the data looks good, you have to know something about the diagnostic strategy the OBD II system uses to determine a good reading from a bad one. This is where things can get real complicated if a sensor or other component apparent...
To make sense of large amounts of profiling data, and to determine when a compound is sufficiently selective, there is a need for a proper quantitative measure of selectivity. Results Here we propose a new theoretical entropy score that can be calculated from a set of IC50 data...
The free drug concentration in the tissues may still be very useful, which is very difficult to measure. Its use for lead drug candidate selection may still be debatable. Several methods are available to determine free unbound drugs (fu) in the tissues, among which equilibrium dialysis is the...
These logo plots therefore provide a way to both identify antigenic mutations and quantify the magnitudes of their effects in a way that is directly comparable across antibodies. Our goal is to determine the fraction of mutant virions that survive antibody neutralization for all mutations to HA. ...
Results Embryonic gut mesenchyme stiffness during colonization by enteric neural crest cells. We devised a simple uniaxial tensile test based on glass fiber deflection25,26 to determine the bulk elastic modulus of chick embryonic gut (Fig. 1a,b, detailed description in Materials & Methods). ...
The homogenate was centrifuged at 6000 × g for 10 min at 4 °C, and the resulting supernatant was used to determine the total protein concentration and the MDA, GSH, and PC activity (see Supporting Information). 2.9. Statistical analysis Tests for significance between treatments were determined...
The partial nucleotide sequence of VP6 regain was done to determine the relation with other registered Rotavirus strains in GenBank. The sequencing was adopted from the forward direction at Macrogen3730XL6-1518–009, Korea. 2.3.4.8. Extraction protein and RNA of HRV HRVq1 isolate An Eppendorf co...
Methods: MTT test was applied to determine the IC50 of docetaxel and the combined effect of B7-H7 siRNA and docetaxel on the viability of the MKN-45 cells. To determine B7-H7, BCL-2, BAX, and caspase-3-8-9 genes expression, qRT-PCR was performed. Furthermore, flow cytometry was ...