For some reason, simply solving the equation for X (concentration) using the absorbance value of Y does not seem to make sense for me. Would someone be willing to walk me through the calculations needed to dete
When conducting a scientific experiment it is necessary to know that you have the correct concentration of the different chemicals involved. It is also important to be able to calculate concentration in order to determine how much of a reactant has been used up in a reaction or how much produ...
In this video, you will learn how to determine the color of a sample with our UV/VIS Excellence spectrophotometers. We will demonstrate this analysis with the color determination of an electrolyte. As decomposition progresses, the color of the electrolyte turns yellowish. Therefore, we will ...
How would the presence of a white solid in a solution effect the solution's absorbance? Explain how sulfate ion (as an example of an anion) can be analyzed via EDTA titration. In a TLC experiment, how to determine which R_f values for the spots ...
Lack of spectral scanning—Filter-based microplate readers cannot be used to run full spectral scans to determine absorbance and emission spectra and peaks for novel dyes or to characterize new assays. Lower spectral resolution—Filters have a relatively wide wavelength range and allow a broad spectr...
There are two standard methods accepted for determining the color of these products, one related to the European Brewery Convention (EBC) and the other is the Standard Reference Method (SRM). Both methods are based on absorbance, but in case of the more and more popular fruit beers these ...
Answer to: How to calculate the slope, absorbance, and concentration of a best fit line once absorbance and concentration values are plotted on a...
Since it is very difficult to assay for cutting near the end of DNA, the effectiveness of compensation with extra enzyme units or increased incubation time is difficult to determine. Use of proofreading enzymes in PCR may also complicate the situation as these enzymes are capable of degrading ...
Serial dilutions also offer benefits to screening-related applications. The detection of minimal concentrations close to the limit of detection (LOD) is only possible when sequentially diluting a known concentration with a fixed factor. For example, in microbiology,...
Hazard and risk assessment of nanomaterials (NMs) face challenges due to, among others, the numerous existing nanoforms, discordant data and conflicting results found in the literature, and specific challenges in the application of strategies such as grouping and read-across, emphasizing the need for...