The possibility to detect and quantify protein-protein interactions with good spatial and temporal resolutions in live cells is crucial in biology. Number and Brightness is a powerful approach to detect b...
One additional possible consideration is that the ECSIS assay can be more informative compared to other assays, such as AB and CFE in this case, as it can monitor the cells in real time, making it a useful tool to evaluate the dynamics of cellular responses and to detect relevant time ...
GroEL promotes protein folding6,25. Therefore, our kinetic model of GroEL should reflect this in the fit parameters\({\varphi }_{F}\)and\({\varphi }_{M}\)(Eq.7and Supplementary Fig.2) that correspond, respectively, to the fraction of client protein molecules that partition either into t...
To answer these questions will require studies in eukaryotic cells with techniques sensitive enough to detect mistranslation and its effects in theabsenceof fidelity-altering drugs. References M. Bucciantini, E. Giannoni, F. Chiti, F. Baroni, L. Formigli, J. Zurdo, N. Taddei, G. Ramponi,...
The detection limit is around 100 nM Zika NS1 protein [22]. These complex emulsions have also been shown to detect analytes easily such as for example caffeine (Figure 2d) [23]. The Janus droplets are stabilized with a specific caffeine-sensitive surfactant. This specific surfactant can bind ...
4). The sensor was produced using graphene nanosheets embedded with a specific antibody against the S protein of SARS-Co-V-2. Samples of antigenic protein, cultured virus, and a nasopharyngeal swab from patients with COVID-19 were used for system validation. The device was able to detect ...
Using a novel lactadherin-based compound, we detect phosphatidylserine (PS)-positive procoagulant platelets in the inflamed vasculature. We identify exposed collagen as the central trigger arresting platelets and initiating subsequent PA in a CypD- and TMEM16F-dependent manner both in vivo and in ...
A new antimicrobial-resistance gene, VCC-1, a ß-lactamase gene, has been discovered in benign close relatives of virulent Vibrio cholerae, which causes cholera. Now, a team of Canadian researchers has found a way to block the VCC-1 enzyme, which disab
A new diagnostic imaging tool in PET/computed tomography (CT) is the use of radiolabeled fibroblast activation protein inhibitor (FAPI). SGHI uses high-frequency sound waves to penetrate deeper into the tissue, providing a more detailed view of the tumor microenvironment. Artificial intelligence ...
Microfluidics-based immunofluorescence techniques combine spatial and spectral encoding approaches and can detect up to 42 secreted proteins from a single cell [183]. Microfluidic western blotting integrates electrophoretic separations and multiple-cycle immunoassays, thereby allowing protein measurement in single...