How is phosphorylated protein detected in Western blot? To quantify a phosphorylated protein,fluorescent conjugated antibodiescan be used to detect phosphorylated and total proteins on the same blot in a process called multiplexing. How is tyrosine phosphorylation detected? The development of monoclonal an...
Human cardiac myosin binding protein C (cMyBP-C, gene name MYBPC3) is a 1274 residue protein which belongs to the immunoglobulin superfamily and contains 3 fibronectin type-Ill domains and 8 Ig-like C2-type (immunoglobulin-like) domains [1]. MyBP-C modulates muscle contraction by regulating ...
Protein post translational modifications—includes cleaved and phosphorylated proteins RNA—including both miRNA, and mRNA transcripts Cell health status—detection of apoptotic cells or cell death Cell cycle status—providing a powerful tool to assess cells in G0/G1 phase versus S phase, G2, or pol...
This allows to resolve proteins of very similar mass, notably unmodified and small molecular mass post-translationnally modified (e.g. phosphorylated) variants of a same protein. IEF is often used upstream of SDS-PAGE in so-called 2D gel electrophoresis [21] . Capillary electrophoresis (CE) is...
Introduction Protein destruction may seem like a strange way for a cell to control its growth. The entire process by which cells multiply is based on synthesis- the duplication of almost every molecule in the cell. If protein synthesis is one of the foundations of growth, then why are so ...
to detect perturbations in essential cellular systems [3]. This ability is advantageous for germline-encoded receptors, as it enables the detection of a diverse range of pathogens. However, these sensors are also more likely to be aberrantly activated under sterile conditions that trigger cellular ...
Previously, we could not immunodetect nucleolar RNA-binding protein nucleophosmin/B23 in mole vole sex bodies54. In this study, we demonstrate that the Nlbs are SUMO-1- and DAPI-positive sites and seem to have a chromatin nature. The mono-ubiquitinated histones H2A (ubiH2A) and H2B play ...
and what to do about it, I argue that when effects are small, black-box randomized trials are simply not going to work. On one hand, you’ll need huge sample sizes to reliably detect small effects; on the other hand, the world is changing and once you get that huge sample size...
(5-micron) resolution, as well as its capacity to support multiplexed detection. The Sapphire FL is widely used for looking at total and modified (e.g., phosphorylated) versions of the same protein while also detecting an in-well standard to ensure accurate quantification across an entire ...
This is achieved by first treating the cleaned up PCR products with T4 Polynucleotide Kinase (if the primers have not already been phosphorylated). The ends will already be blunt if a proofreading thermostable polymerase such as Pfu was used or may be treated with T4 DNA Polymerase to polish...