Many phospho-specific antibodies are quite sensitive and can readily detect the phosphorylated protein in a routine sample (e.g., 10-30 µg whole cell extract). Because the measured levels of a phospho-protein may change with treatment or through gel loading errors, researchers often utilize ...
How is phosphorylated protein detected in Western blot? To quantify a phosphorylated protein,fluorescent conjugated antibodiescan be used to detect phosphorylated and total proteins on the same blot in a process called multiplexing. How is tyrosine phosphorylation detected? The development of monoclonal an...
To provide a new method for detecting an abnormal condensation of chromatin by identifying phosphorylated peptide required for chromosome condensation, using an early chromosome condensation which can induce chromatin condensation alone from an interphase cell and by using the degree of phosphorylation of ...
This is achieved by first treating the cleaned up PCR products with T4 Polynucleotide Kinase (if the primers have not already been phosphorylated). The ends will already be blunt if a proofreading thermostable polymerase such as Pfu was used or may be treated with T4 DNA Polymerase to polish...
Keywords: NLRP3; Inflammasome; Mechanism; Localization; Structure; Regulation Cellular & Molecular Immunology (2022) 19:1201–1214; https://doi.org/10.1038/s41423-022-00922-w INTRODUCTION A vital function of the immune system is to detect alterations in tissue homeostasis and respond in a context...
The S910PPP motif, when phosphorylated by Erk 1/2 on serine 910, becomes a substrate for the peptidyl-prolyl cis/trans isomerase PIN1. PIN1 recruits the protein tyrosine phosphatase PTP-PEST, which dephosphorylates FAK Y397, leading to increased FA turnover and tumour cell invasiveness (Zheng...
What is a protein that detects a signal molecule and performs an action in response called? The conformational state of a protein can be diagnosed from which type of protein? How is E2F protein regulated by cyclins and Rb protein? UV absorption is commonly ...
1). Vital to cell progression is O-GlcNAcylation. Simply, O-GlcNAc is the attachment of a single N-acetylglucosamine sugar to serine and/or threonine amino acids in nuclear, cytoplasmic, or mitochondrial proteins (1). Like most posttranslational modifications, O-GlcNAc regulates protein function,...
Previously, we could not immunodetect nucleolar RNA-binding protein nucleophosmin/B23 in mole vole sex bodies54. In this study, we demonstrate that the Nlbs are SUMO-1- and DAPI-positive sites and seem to have a chromatin nature. The mono-ubiquitinated histones H2A (ubiH2A) and H2B play ...
Microtubules form the basis of primary cilia, organelles located on the surface of most postmitotic mammalian cells. In their capacity as signaling hubs and sensory organelles, primary cilia have the ability to detect and react to mechanical and chemical stimuli present in the extracellular space.17...