A method of culturing mammalian cells under conditions sufficient to produce recombinant protein in a liquid culture medium containing treated bovine serum albumin (BSA) at or above 0.1 g / L. Culturing mammalian cells containing nucleic acid encoding the recombinant protein, wherein the treated BSA...
To keep cells in optimal culture conditions and actively growing, it is necessary to renew the growth medium and to subculture them at regular intervals. Change of culture medium can take place several times in the log phase dependent on the cell type. The best time to subculture cells is be...
Cell clumping occurs when cells aggregate in a culture and begin to block each other from resources. Learn more here.
How did cells make the journey, one we take so much for granted, from their origin in living bodies to something that can be grown and manipulated on artificial media in the laboratory, a substantial biomass living outside a human body, plant, or animal? This is the question at the heart...
cells reaches a plateau referred to as the stationary phase (Figure 4). In this phase, the number of new cells created by cell division is now equivalent to the number of cells dying; thus, the total population of living cells is relatively stagnant. The culture density in a stationary ...
As the living standard of the Chinese people is rising, we look forward to further changes in peoples diet. In the future, the proportion of milk and meat is likely to go on increasing and the proportion of fruit and vegetables is likely to increase as well. ...
& Roux, A. Epithelial cells adapt to curvature induction via transient active osmotic swelling. Dev. Cell 57, 1257–1270.e5 (2022). Article Google Scholar Gómez-González, M., Latorre, E., Arroyo, M. & Trepat, X. Measuring mechanical stress in living tissues. Nat. Rev. Phys. 2, ...
While some are free-living and have no impact on human beings (and other animals), some are either beneficial or harmful making it necessary to study and understand them. This has not only made it possible to develop treatments for diseases caused by specific species of fungi, but also ...
coli cell itself is the product of billions of years of evolution, so it is complex and intricate -- much more complex than the first living cells. Even so, the first living cells had to possess: A cell wall The ability to maintain and expand the cell wall (grow) The ability to ...
Evidence from animal studies suggests that stimulating the brain can stop cells from shrinking and can even increase brain size in some cases. Studies show that rats living in enriched environments with lots of toys and challenges have larger outer brains with larger, healthier brain cells. And an...