RRF is generally used to compare the peaks of impurities to the primary peak or peaks of the substance you are analyzing. The separation of components of the substance also depends on vapor pressure, polarity of the components, temperature of the gas chamber and the amount of material you init...
Interestingly, performing liquid separations at low pH (by addition of formic or acetic acid) is statistically better since sharper peaks can be obtained on the chromatogram. Formic acid and acetic acid are acid additives that are compatible with mass spectrometry detection, meanwhile, at the same ...