OmicsBox allows you to performNCBI Blastsearches using acloud systemfor high-performance computing. This system runs jobs in parallel and autoscales depending on demand. To control usage we use so-called CloudUnits. These units represent computation time (CPU seconds) on the AWS infrastructure. At...
Primer-BLAST is NCBI’s free online primer design platform which designs PCR primers using the Primer3 system and simultaneously checks the likelihood that the primers will bind to unspecific regions of the organism’s genome via the BLAST algorithm. ...
In the future, it will be interesting to see ifdatZandpurZgenes are sufficient for transferring 2-aminoadenine to the genomes of other organisms. Methods Identification of genes of interest The genomic sequence of cyanophage S-2L was obtained from NCBI’s database (AX955019). Potential ORFs ...
Despite all the medical advancements and discoveries over the past several decades, approximately 30% of all known diseases can be currently treated with available pharmacological agents. However, the discovery of new pharmaceutical drugs is one of the m
首先在 NCBI 在线软件 ORF finder ( https://www.ncbi.nlm.nih.gov/orffinder/ ) 分析基因 的开放阅读框(open reading frame,ORF)和翻译出 的蛋白序列;通过 NCBI 数据库的在线软件 blast (https://blast.ncbi.nlm.nih.gov/)寻找与 LjPDS 蛋白 序列相似的其他物种的 PDS 蛋白序列并下载; DNAMAN 6.0 ...
It is commonly assumed that a heterotrophic ancestor of the supergroup Archaeplastida/Plantae engulfed a cyanobacterium that was transformed into a primary plastid; however, it is still unclear how nuclear-encoded proteins initially were imported into th
Quantization of perfectly matching nucleotide motifs was done using the BLAST Web-server at NCBI [44]. Full size image For different human adenoviruses, we identified 47-106 perfectly matched 21 nt-long motifs, 16-44 22 nt-long, 4-19 23 nt-long, 0-8 24 nt-long, 0-6 25 nt-long, 0...
BLAT—the BLAST-like Alignment Tool. Genome Res. 12, 656–664 (2002). Article CAS Google Scholar Stein, L. Genome annotation: from sequence to biology. Nature Rev. Genet. 2, 493–503 (2001). Article CAS Google Scholar Pruitt, K.D. & Maglott, D.R. RefSeq and LocusLink: NCBI ...
Cite this protocol Gouw, M.et al.(2020). How to Annotate and Submit a Short Linear Motif to the Eukaryotic Linear Motif Resource. In: Kragelund, B.B., Skriver, K. (eds) Intrinsically Disordered Proteins. Methods in Molecular Biology, vol 2141. Humana, New York, NY. https://doi.org...
U-Lampyristoxin-Ln6 is another putative toxin with similarity to a compound from scorpion venom. In this case, the BLAST hit was annotated as beta toxin, which indicates a potential modulation of the inactivation process of sodium channels. Surprisingly, U-Lampyristoxin-Ln7 is the only putative...