If you know the concentration of enzyme sites you've added to the assay (Et) then you can calculate the catalytic constant Kcat. It is defined to equal Vmax/Et. Vmax and the Y values (enzyme velocities) are expressed in units of concentration per time, and Et must be entered in those ...
Beyond Vmax and Km: How details of enzyme function influence geochemical cyclesSteen, Andrew
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Divide 1 by the result from Section 2, Step 3 to calculate the actual value of Vmax. Determine the concentration of the enzyme in the original assay (see the raw data). Note: the enzyme concentration is the same for all of the test tubes; only the substrate concentrations vary in the a...
One Enzyme, One Reaction In most situations, decreasing enzyme concentration has a direct influence on enzyme activity because each enzyme molecule is able to catalyze only one reaction at a time. The molecule to which an enzyme binds is called a substrate. In general, one enzyme binds to one...