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the maximum reaction velocity for the enzyme. Note: if the linear regression model takes the form "[Inverse Vo] = m [Inverse Substrate Conc.] + b," then the value of "b" would be the y-intersect. Divide 1 by "b" to calculate the inverse of Vmax....
If you plot enzyme velocity as a function of subtrate concentration, you can fit the data to the Michaelis-Menten equation to determine the Kmand Vmax. The Vmaxis the maximum enzyme velocity extrapolated out to very high concentrations of substrate. It is expressed in the same units you used ...
where k is a rate constant specific to the reaction. The concentrations of the reactants may be raised to an exponent (typically first or second power).