A loading control is a protein that is highly and constitutively expressed in your sample and that is used as a positive control in your western blot experiment. The most important reason for using a loading control is to ensure the reliability of your data and conclusions, as it helps demons...
Hemoglobin is the protein in red blood cells that binds to oxygen (O2) in the lungs and transports it to tissues that require it for respiration. An outstanding property of hemoglobin A (HbA) is that it participates in cooperative binding with O2. This essentially means that at very high O2...
Explain how to calculate (2.00 x 10-3) - (1.44 x 10-3). Explain how a titration works. What information can you determine from a titration, and what information is necessary to begin with? Define equivalence point and ...
1. Explain the chemical information that can be determined about an acid using the equivalence point and half-equivalence point of a titration curve. 2. Describe when you can use the Henderson- Hasse How might you use mass spectrometr...
Briefly, the recombinant α-syn protein was dissolved in 20 mM Tris and 100 mM NaCl to have a final concentration of 140 μM (pH 7.4). After dissolving, the protein solution was filtered through 100 kDa filter (MERCK, MRCFOR100). The filtrate was transferred to a low-protein binding ...
(1)standardproteinsolution:usingstandardcrystallinebovineserumalbumin(BSA)orstandardcasein,preparationofstandardproteinsolution10mg/ml,availableBSAconcentrationof1mg/mlA2800.66tocorrectitspurity.Ifnecessary,standardproteincanalsobeusedtodeterminethecontentofproteinnitrogenbymicroKjeldahlmethodinadvance,calculateitspurity,and...
How many kilograms of salt must be added to 20 kg of an 80% salt solution to produce an 85% salt solution?Answer and Explanation: First we need to determine how much salt and solvent we have: 0.8 * 20 = 16 kg salt Since 80% is salt, 20%...
specificity. This method allows the free energy of RNA/DNA duplex formation to be calculated for both off-target and target sequences. Along with computer modeling of CRISPR–Cas9 activity [23], actual measurement of the interaction energy is possible through isothermal titration calorimetry (ITC) ...