However, DNA is not the only molecule that can absorb UV light at 260nm. Since RNA also has a great absorbance at 260nm, and the aromatic amino acids present in protein absorb at 280nm, both contaminants, if present in the DNA solution, will contribute to the total me...
The UV-Vis light passes through the sample and reaches the detector. The transmittance is measured and used to calculate the absorbance. An absorption spectrum is obtained with absorbance plotted on the vertical y-axis and wavelength plotted on the horizontal x-axis. Lab Manager What you will ne...
The elution of protein was monitored by UV absorbance at 280 nm. Different fractions were visualized by SDS-PAGE analysis, and fractions of interest (oligomer) corresponding to the void volume peak were aliquoted (500 μL), snap-frozen, and stored at −20 °C. 5.3. Preparation of DA-...
The velocity of the Brownian motion is defined by a transla- tional diffusion coefficient that can be used to calculate the hydrodynamic radius, i.e. the radius of the sphere that would diffuse with the same rate as the molecule of interest. This is done by measuring, with an autocorre-...
In RNAs, m6A is thought to primarily function by recruiting proteins to specific modified sites (reviewed in refs.3,4,5). However, there is also growing evidence that the modification can impact a range of biochemical processes by changing the behavior of the methylated RNAs10,11. For example...
There’re indeed some formula to calculate the paper weight under different units. However, the easiest solution would always be going to some website with the readily available converters. Click thelinkhere and you will have the tools for very quick conversion. ...
In general, how to determine which of several peaks in a chromatogram obtained for What is the best way to analyze a hypothesis? What is the necessary assumption to calculate K_{obs} using non-graphical methods? Transform the compound in a fisher projection and label R or S. ...
Although the cofactor loading of the D8A mutant was lower (B40%), UV-visible absorbance properties were also consistent with the presence of a [4Fe–4S] cluster (Supplementary Fig. 7c). To confirm the nature of the clusters bound in D8C and D8A variants, ESI-mass spectrometry under non...
ΦI) with the value for the partition constant between oil and water in the absence of emulsifier (PWO = PWI/POI), it is possible to calculate the values of PWI and POI by solving two equations for two unknowns. Details on these determinations and simplifications employed for determining ...
In this context, our study aimed to evaluate the impact of discoloration of porcine cruor hydrolysate on its resulting antimicrobial activities. The specific objectives of the present study were (1) to explore the peptide profiles of the raw and discolored porcine cruor hydrolysates, (2) to identi...