ImageJ software (NIH, USA) was used to process the acquired TEM images to calculate mean size and size distribution of the single particles in the NMs preparations. For the anisotropic NMs (e.g. Ag nanowires, T
In ImageJ, there are two methods of adding a scale bar. The first method is taking an image of the scale bar (such as a ruler or micrometer), selecting the straight line selection tool, and drawing a line over the scale to define a known distance. Select the "Analyze" menu, and ch...
knowledge, we are able to develop a method to quantify MP based on the deviation of model parameters from reference class I and II parameter values. In short, we assume that a class I or II parameter follows the normal distribution in which the mean represents the reference value. The more...
Functionality being removed or changed The regionprops function uses new equation for circularity Behavior change 5-4 Starting in R2023a, the regionprops function uses a new equation to calculate circularity. The new equation removes a bias that caused the computed circularity to be too high for ...
Scale bar: 2 μm. (B) Snapshots of a DiD-labeled stdDENV, Ab-stdDENV and Ab-prMDENV particle at different time points post-infection. Membrane fusion is observed as a sudden increase in fluorescence intensity. The image was artificially modified to show the differences in fluorescence ...
From there, you can calculate the actual pixel size of the microscopy images using the following formula: Actual Pixel size = (CCD Pixel x Binning) / Lens Mag x C mount x Objective Mag. Scale bars in ImageJ In ImageJ, there are two methods of adding a scale bar. The first method is...
However, it was not possible to fit the data and calculate the kinetic rate constants and binding affinities for 9029. Both antibodies showed a slow dissociation of monomers, as evidenced by the fact that the dissociation portion of the sensorgrams (after 120 s) returns slowly to the baseline...
In Ref. [12], we used this approach to fully calculate the pH close to a vesicle submitted to a local injection of acid with a constant flow (see Ref. [12], Supporting material). Hence, we can obtain the spatiotemporal evolution of the membrane deformation by carrying out the integrals...
[28,64]). Following this calibration, the marquee tool in Photoshop was used to calculate the values of the red (R), green (G) and blue (B) colour channels of the coloured area of the dorsal fin and representative, similar sized areas of the body. Estimates of R, G and B are ...
we phenotyped selected leaves by drawing their circumference on a sheet of paper to calculate the leaf blade area, leaf index, and leaf shape index (Figure 1A). The terms used to characterize the shape of the cabbage leaf are derived from the Community Plant Variety Office [18] (Figure 1A...