Explain why the center of the band is used to calculate the Rf value for a cation rather than the leading edge of the band What is the difference between chromatography and spectrography? How can TLC (thin-layer chromatography) and column chromat...
This mark is needed to calculate Rf (Retention factor) values.Spotting a TLCTo spot a TLC, first dissolve the material to be separated in a solvent. Then, draw some solvent into a capillary, and press the capillary onto the TLC place medium. If done correctly, the solvent should all ...