It is possible to calculate the expected average fragment size for a given genomic DNA if the percent GC content of the DNA and the recognition sequence of the restriction enzyme are known. For example, in a genome with 50% GC content and no dinucleotide bias, a four-cutter can be expecte...
Insulin contains fifty-one amino acids. How many base-pairs are required to code the amino acids? Genetic Code: The biological information coded chemically into genetic form by the use of biochemical molecules, known as nucleotides, is called genetic code. All the...
For protein-protein interfaces, where it is possible to predict structures with reasonable accuracy with AlphaFold219(AF2), we further probe the “goodness” of the designed interfaces by estimating their binding strengths and comparing them to wild-type interfaces. For antibody-antigen interfaces, w...
These properties were represented among the 20 descriptors that were used to calculate the PCA and therefore were accounted for in the overlapping scores. It is therefore not surprising to picture these datasets as close in chemical space. In fact, it is clearly visible from the surface map of...
Calculate the change in pH when 37.00 mL of a 0.700 M solution of NaOH is added to 1.00 L of a solution that is 1.00 M in sodium and 1.00 M in acetic acid. What is the overall charge of glutamic acid at a pH of 2 and 9? Why aren't the other amin...
We next asked how it is possible that long-lived misfolded proteins are able to bypass the post-translational cellular chaperone machinery. To address this question we used coarse-grained Langevin dynamics to calculate the dissociation constant between the chaperone GroEL and three distinct conformational...
The conductivity of the protein mixtures was measured with a CO 3000 L conductivity meter (VWR International, Leuven, Belgium) at 20 °C. The measured conductivities were used to calculate the ionic strengths – expressed as the equivalent of a NaCl molar concentration – using Eq. (6) (But...
This was demonstrated by the simultaneous detection of the mutation underlying Miller syndrome by WGS of a family consisting of two affected siblings and their parents with the added bonus of being able to calculate a human intergenerational mutation rate of approximately 1.1 × 10-8 per position ...
It is possible to calculate the expected average fragment size for a given genomic DNA if the percent GC content of the DNA and the recognition sequence of the restriction enzyme are known. For example, in a genome with 50% GC content and no dinucleotide bias, a four-cutter can be expecte...
N6-methyladenosine (m6A) is a post-transcriptional modification that controls gene expression by recruiting proteins to RNA sites. The modification also slows biochemical processes through mechanisms that are not understood. Using temperature-dependent (