functional state by the last time point. The percentage of molecules that did not fold ranged from a low of 6% to a high of 70% (Table1, Fig.1aand Supplementary Data1). Since structure equals function, these percentages indicate there is an appreciable fraction of protein...
These properties were represented among the 20 descriptors that were used to calculate the PCA and therefore were accounted for in the overlapping scores. It is therefore not surprising to picture these datasets as close in chemical space. In fact, it is clearly visible from the surface map of...
It is possible to calculate the expected average fragment size for a given genomic DNA if the percent GC content of the DNA and the recognition sequence of the restriction enzyme are known. For example, in a genome with 50% GC content and no dinucleotide bias, a four-cutter can be expecte...
Identify the type of high energy bond in the intermediate of the glyceraldehyde-3-phosphate dehydrogenase reaction. Why is it important? Which buffer system is the best choice to create a buffer with pH=7.20? For the best system, calculate the ratio of the masses of the ...
The conductivity of the protein mixtures was measured with a CO 3000 L conductivity meter (VWR International, Leuven, Belgium) at 20 °C. The measured conductivities were used to calculate the ionic strengths – expressed as the equivalent of a NaCl molar concentration – using Eq. (6) (But...
MRI technology is founded in NMR Spectroscopy and relies on the basic principles of the technique to generate diagnostic images. In the food and beverage industry, NMR Spectroscopy is used to determine the structure of proteins, analyse amino acids and detect naturally occurring pigments such as ...
This was demonstrated by the simultaneous detection of the mutation underlying Miller syndrome by WGS of a family consisting of two affected siblings and their parents with the added bonus of being able to calculate a human intergenerational mutation rate of approximately 1.1 × 10-8 per position ...
Error bars in (B), (C) and (D) represent SEM of triplicate data used to calculate the representative IC50 values in (A). (E) Location of BimBH3 ‘h0’ and h1 residues in the proposed alternative Bax binding site, the α1/α6 interface (Gavathiotis et al., 2008). The model ...
N6-methyladenosine (m6A) is a post-transcriptional modification that controls gene expression by recruiting proteins to RNA sites. The modification also slows biochemical processes through mechanisms that are not understood. Using temperature-dependent (
While these models have proven to be effective when describing small-scale/ narrowly defined conformational transitions, the vast phase space associated with accommodation makes it computationally intractable to calculate the free energy. In contrast, through the use of simpler models, one may evaluate ...