We next asked how it is possible that long-lived misfolded proteins are able to bypass the post-translational cellular chaperone machinery. To address this question we used coarse-grained Langevin dynamics to calculate the dissociation constant between the chaperone GroEL and three distinct conformational...
These properties were represented among the 20 descriptors that were used to calculate the PCA and therefore were accounted for in the overlapping scores. It is therefore not surprising to picture these datasets as close in chemical space. In fact, it is clearly visible from the surface map of...
The measured conductivities were used to calculate the ionic strengths – expressed as the equivalent of a NaCl molar concentration – using Eq. (6) (Butré, Wierenga, & Gruppen, 2012).(6)Ionicstrength(NaClequivM)=1.02⋅10−5⋅conductivity(μs.cm−1) 2.2.4. Mineral composition The...
Which buffer system is the best choice to create a buffer with pH=7.20? For the best system, calculate the ratio of the masses of the buffer components required to make the buffer. HC2H3O2/KC2H3O2 H If you were conducting an experiment with pepsin, which has optimal e...
It is possible to calculate the expected average fragment size for a given genomic DNA if the percent GC content of the DNA and the recognition sequence of the restriction enzyme are known. For example, in a genome with 50% GC content and no dinucleotide bias, a four-cutter can be expecte...
MRI technology is founded in NMR Spectroscopy and relies on the basic principles of the technique to generate diagnostic images. In the food and beverage industry, NMR Spectroscopy is used to determine the structure of proteins, analyse amino acids and detect naturally occurring pigments such as ...
(D) Inhibition curves for peptides showing negligible inhibition of the binding of BaxΔC21 to BimBH3 peptide (titration from 2.93 nM to 3 μM). Error bars in (B), (C) and (D) represent SEM of triplicate data used to calculate the representative IC50 values in (A). (E) Location ...
We next asked how it is possible that long-lived misfolded proteins are able to bypass the post-translational cellular chaperone machinery. To address this question we used coarse-grained Langevin dynamics to calculate the dissociation constant between the chaperone GroEL and three distinct conformational...
To this end, we used our simplified model to calculate the free energy of aa-tRNA elbow accommodation with EF-Tu bound to the ribosome in its post-hydrolysis, pre-Pi-release conformat- ion21,29. Relative to the calculations described in Fig. 1, the only difference in this set of ...
N6-methyladenosine (m6A) is a post-transcriptional modification that controls gene expression by recruiting proteins to RNA sites. The modification also slows biochemical processes through mechanisms that are not understood. Using temperature-dependent (