1021 A New Protocol for Determining Intracellular Concentrations of Antitumor Compounds – How to Calculate a Real and Effective IC50 - European Journal of Cancerdoi:10.1016/S0959-8049(12)71636-2D. HeviaA. Rodriguez-GarciaH.M. Cimadevilla
However, the dose-response curve may not extend between the controls (the NS controls are considerably lower than the Bottom plateau) as seen below. So, how do we calculate the IC50? The relative IC50 is estimated by considering the Bottom and Top plateaus obtained by implementing the 4PL mo...
(D) Inhibition curves for peptides showing negligible inhibition of the binding of BaxΔC21 to BimBH3 peptide (titration from 2.93 nM to 3 μM). Error bars in (B), (C) and (D) represent SEM of triplicate data used to calculate the representative IC50 values in (A). (E) Location ...
This approach involves generating viral libraries containing all mutations to the protein of interest, selecting these viruses with or without antibody, and using an accurate deep-sequencing method to determine the relative frequencies of each mutation. These frequencies can be analyzed to calculate the...
Indirect response models were used to express the results. as an inhibitory percentage (IP) and a titer (TCID50/mL), as reported by Lelešius et al., (2019). The subsequent formula was applied to calculate the inhibition.(2)%InhibitionPercentageIP=1-T/C×100Where C is the antilog of...