What is the delta-delta Ct method? The delta-delta Ct method, also known as the 2–∆∆Ctmethod, is a simple formula used in order to calculate the relative fold gene expression of samples when performing real-time polymerase chain reaction (also known as qPCR). The method was devised...
4. Calculate delta Ct values Next, we need to calculate∆Ctseparately for each gene in each sample. To do this, simply subtract the newly created ‘Control average‘ (now acting as the calibrator/reference) value from the ‘Average Ct‘ of each sample (including all of the control samples...
To do this, simply subtract the Ct values of the antibodies of interest from the ‘adjusted input‘ Ct value. I have done this in a new column called ‘Delta Ct‘. 4. Finally, we can calculate the % of input value based on the delta Cts. To do this, we do 2 to the power of ...
Plant height was measured every 3 days during the bolting period to calculate the average stem height. 4.10. Light Microscopic Analysis Dry seeds from OX-PtCP5 and wild-type plants were vacuum-infiltrated for 1 h with a fixative that consisted of 30% formaldehyde, acetic acid and 50% ethanol...
When the samples were treated with propiodium monoazide (PMA) to test for cell integrity followed by qPCR, the number of C. antarcticus cells was equally undamaged under all conditions (~66%), while C. minterii showed the highest number of undamaged cells at 0.1% UV (51.12 ± 3.34%) ...