Answer to: How to calculate the slope, absorbance, and concentration of a best fit line once absorbance and concentration values are plotted on a...
When conducting a scientific experiment it is necessary to know that you have the correct concentration of the different chemicals involved. It is also important to be able to calculate concentration in order to determine how much of a reactant has been used up in a reaction or how much produ...
How to calculate the distribution coefficient in caffeine? Discuss the nature and causes of deviations from Beer's Law in spectrophotometric How was UV-visible spectrophotometer used to relate the absorbance to the concentration of the aspirin? What is the absorbance if you measure the potassium ch...
So, you'll want to plug the absorbance value for each unknown into the equation in place of y, then calculate x. You can use the spreadsheet program to do this calculation for you and make it quicker. You have now calculated the concentration of the chemical of interest in three of your...
Re: How to determine the concentration of Pb from Absorbance Value? « Reply #1 on: October 12, 2011, 11:19:39 AM » You need to calculate the total amount of lead and divide by the original sample weight.You can determine the lead concentration from your slope calculation. There are...
How to Perform Fixed Wavelength Measurements with UV/VIS Excellence Spectrophotometers In this video, you will learn how to set up a method for measuring the absorbance at several wavelengths and how to calculate multiple results. For example, we have chosen the analysis of olive oil samples...
According to the absorbance value of the tested sample, the corresponding protein quality was found on the standard curve, and the protein concentration of the sample solution was calculated. Because of the different amounts of tyrosine and phenylalanine in different proteins, the depth of coloration...
It is possible to calculate the expected average fragment size for a given genomic DNA if the percent GC content of the DNA and the recognition sequence of the restriction enzyme are known. For example, in a genome with 50% GC content and no dinucleotide bias, a four-cutter can be expecte...
In addition, Synopsys offers high-end scattering measurement instruments for your lab to measure BSDF, which could be used for TIS computation. For example, the Synopsys REFLET 180S is able to measure BSDF in order to extract/calculate TIS from these measurements. The accuracy depends on the re...
Explain how to calculate absorbance change per minute in ONPG enzyme activity. What is the model of enzyme activity that explains how a particular enzyme will only fit with one particular type of substrate? Why might a glucose test strip be used to determine the enzymatic...