Integrated meta-omics approaches hold the potential to resolve the fundamental niches and realized niches of microbial populations in situ15. The former represents the exhaustive inventory of resource ranges and conditions sustaining viability in the absence of environmental stress, competition, or predation...
Mass spectrometry tools enable the analysis of multiple molecules from a complex sample; those identified chemical compounds can be then correlated to a relevant phenomenon or biological question. Thus, applications of MS in mycological studies are not restricted to the identification of chemical compound...
In addition, each spectrum was manually checked to ensure that the monoisotopic peaks were correctly identified. 4.4. Top-Down and Bottom-Up Proteomics The proteome of midgut secretions of larval stages of L. noctiluca was analyzed using both top-down and bottom-up strategies as described in [...
A denaturation-then-titration strategy (assigning the protein under harsh conditions and then titrating to physiological conditions; see Supplementary Information) was used to assign the chemical shifts of the protein. In addition to standard pulse sequences (HNCO, HN(CA)CO, HNCA, HN(CO)CA, ...