coli templating machinery can be recruited to assemble the Salmonella MS-ring (~95% identical) and is also unlikely to underlie the stoichiometric difference. Nevertheless, we did not test conditions proposed to affect stoichiometry, which would be required to distinguish between an adaptive and a ...
Capping protein is known to contribute to branched actin network assembly in several ways. First, it prevents the elongation of filaments beyond a critical length at which they would easily buckle under load6,19,20. Second, it rapidly stops the non-productive elongation of filaments distant from ...
–Pro tip: Remove the clips for the fire pan.Remove the lid, cooking grids, water pan, and fire pan. Remove the clips where the fire pan normally sits. Measure the height from the bottom of your fire pan to the ground, and use a brick or paver stone to hold it at the correct hei...
To assemble dynein-dynactin-BICD2N (DDB) complexes for single molecule assays, 1 μL of fully purified TMR labeled wild-type dynein (wtDyn) or phi-interface mutant dynein (mtDyn) at a concentration of 200nM was mixed with 2ul of dynactin and 1ul SNAPf-BICD2N to achieve a molar ratio...
These proteins assemble into pairwise aligned filaments, called rodlets, on the outer surface of the spores and are spaced ~20 nm apart (Table S2). Until now, the function of the rodlets has remained elusive [30]. Interestingly, the diameter of the bacterial flagellar filament is also ~...