Anneal after every second round (both sides of the billet). 2.4 Begin Shaping the Billet Once the billet is reduced to the desired width, it’s time to give it some shape. 2.5 The Shaping Process When the billet is “rounded” using a hammer, file off the soldering that was added befo...
When produced 3′ overhangs from both sides of DSBs have some complementary nucleotides, they can simply anneal to each other. After trimming the remaining ends, re-ligation occurs. However, the precise molecular mechanisms underlying aNHEJ in plants remain unclear [153]. The list of NHEJ-...