Here, we used STORM super-resolution microscopy and single-particle-tracking (SPT) experiments to analyze the differential cell membrane distribution and diffusion behavior of PS, ecto-CRT, and CD47 on adherent HeLa cells. We compared viable cells to cells in early apoptotic stages induced by UVB...
. . . . 2-2 Image Quality: Analyze additional test charts . . . . . . . . . . . . . . . . . . . . . . 2-2 Volume Visualization: Label volumes using 3-D point and line annotations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ...
Since trafficking of the different intracellular compartments is controlled by Rab GTPases44, we used a set of fluorescently-labeled Rab dominant-negative mutants (DNM) to analyze which of these GTPases is required for viral transport. Fluorescently-labeled dextran was used as a control to ...
One central question of centromere biology is how is CENP-A deposited at centromeric chromatin. Members of a protein family named Scm3/HJURP (Holliday Junction Recognizing Protein), conserved from yeast to humans, have been proposed to act as CENP-A specific chaperones in yeast and human cells ...
The main tasks of the simulation are: to communicate by e-mail to the colleagues of the intentions about the study, to search and collect literature on web, to search and select proper images, to collect and analyze data, to write the manuscript, to choose and edit the iconography, and ...
The caudally-migrating (green) and rostrally-migrating (red) ENCCs adhered to each other to form chains of intermingled cells. It was not possible to analyze the effects of collisions between ENCCs migrating in opposite directions on individual cell behavior because high levels of cell-cell ...
moment of kinetic network arrest through Latrunculin B, phalloidin and myotrophin/V-1. The latter inhibitor preserves free barbed ends8. To minimize the impact of CP dissociation from capped ends after network arrest, we immediately proceeded to imaging and did not analyze NPF-coated beads longer...
Neutrophils are important effector cells of the innate immune system, traditionally regarded to have a short life span. The goal of this study was to evaluate the effect of the whole blood storage on neutrophil functions, e.g., viability, antimicrobial e
(red) ENCCs adhered to each other to form chains of intermingled cells. It was not possible to analyze the effects of collisions between ENCCs migrating in opposite directions on individual cell behavior because high levels of cell-cell contact prevented us from obtaining pre-collision data for...