3.Evaluate Cre expression based on reporter gene expression through crossbreeding Cre mice and a Cre reporter strain, such as, strains with either a beta-galactosidase (lacZ) or a fluorescent reporter, and analyse Cre recombination in the Cre+ offspring. So how doCrereporter strains workin the ...
molecules during multiple initial rounds of displacement preamplification. These looped DNAs are then further amplified using PCR [149]. MALBAC has been successfully used to analyse single-nucleotide variant (SNVs) profiles in CTCs [150]. LIANTI amplifies T7 promoter-tagged DNA fragments into thousa...
Proteins were visualized with ECL Western blotting reagents (GE Healthcare; Marlborough, MA, USA) according to the manufacturer’s protocol. Flow cytometry analyses were conducted to analyse the expression levels of different MAGEA10 mutants fused with EGFP. Live Cop5-EBNA cells expressing MAGEA10-...