Prepare the solution by mixing 0.477 moles of monosodium phosphate and 0.523 moles of disodium phosphate in a little less than a liter of water. Check the pH using apH meterand adjust the pH as necessary using phosphoric acid or sodium hydroxide. Once you have reached the desired pH, add wa...
Why is ATP used as a source of phosphate for the formation of phosphoester bonds? What types of species are needed to produce a buffer solution? If the tripeptide SGR (serine, glycine, arginine) was dissolved in a buffer that has a pH of 8.5, what would be the net c...
How to prepare 500 ml of a 0.75 M Sodium Phosphate buffer solution at pH 7.5, how would you go about doing that? Describe how you would make 4 liters of a 0.075 M phosphate buffer at pH 7.15 without using a pH meter, if the onl...
aa mixture of 50 volumes of buffer solution prepared by dissolving 4.45 g of disodium hydrogen phosphate dihydrate in 0.1 per cent triethylamine,adjust the pH to 8.5 with orthophosphoric acid and 50 volumes of methanol 溶化的4.45准备的缓冲溶液的50容量混合物g disodium氢磷酸盐二水合物在0.1%三乙基氨...
is a common buffer used in cell culture and histology. The osmolarity and ion concentration of PBS are the same as in human tissues, so it will not cause your cells to shrivel or explode. PBS is 137 mmol NaCl, 2.7 mmol KCl, 10 mmol Na2HPO4, and 2 mmol KH2PO4at pH7.4. Sterile fil...
(5) Adjust the pH value of the buffer system to 3 by adding dilute hydrochloric acid. Cool the mixture to -5°C. After standing and filtering, the crude product is obtained with a conversion rate of 93.5% (based on benzoic acid). ...
Generally, when you buy a new fish for your tank, you bring it home, float the bag for about 20 minutes, and then add the fish to your tank, right?You have not given your fish a chance to adjust.Your fish will experience what is called osmotic shock. While it can take a few ...
Prepare 100 ml of modified RIPA buffer. Weigh 790 mg of Tris base and add it to 75 ml of deionized water. Add 900 mg of NaCl and stir until completely dissolved. Adjust the pH to 7.4 with HCl. Add 10 ml of 10% NP-40. Add 2.5 ml of 10% sodium deoxycholate and stir until the...
What is the role of blood in the regulation of the pH of the body? Describe how the body uses respiration to regulate the PH of the Blood. Explain how breathing controls blood pH. Describe how the respiratory system works to adjust blood pH. Include ...
Adjust the number of enzyme units based on recognition site density. Perform the experimental digest, replacing the experimental DNA with the same quantity of commercial quality DNA (usually lambda DNA). Adjust the number of enzyme units based on recognition site density. Compares activity of the...