How is DNA organized in the nucleus in dividing and nondividing cells? How the structure of DNA contributes to a cell's ability to replicate it? How does the structure of DNA fit its function, especially as it relates to DNA replication?
Describe the steps for DNA processing. How are genes transcribed in eukaryotes? How do cells use the base sequence of a DNA molecule to produce a polypeptide? Describe how eukaryotic DNA is arranged in the cell. During DNA replication, how are the new nucleotides added to the template strand...
Single-nucleotide-resolution chemical RNA structure probing is emerging to explore the folding state of the transcriptome in living cells; the resulting models for how 5′ UTR structures impact translation should be validated by compensatory mutagenesis.Abstract...
Highly conserved protein dimerization domains that occur in many proteins involved in membrane dynamics and act as connecting links between actin dynamics and membrane rearrangements in all eukaryotes. BAR domains preferentially bind to curved membrane regions. Chromatin Complex of genomic DNA with proteins...
In order to maintain genome stability and integrity, cells have evolved a wide variety of DNA repair pathways which counteract different types of DNA lesions, also referred to as the DNA damage response (DDR). However, DNA in eukaryotes is highly organized and compacted into chromatin representing...
protein is expressed and if there is a loading control protein that is also expressed in the same sub-cellular location. For example, if you have prepared lysates consisting solely of nuclear proteins, it would be more applicable to normalize against a DNA-binding protein than a cytoskeletal ...
adaptations is preservation of optimal balances between conformational rigidity and flexibility of macromolecules. Micromolecular adaptations involve several families of organic osmolytes, with varying effects on macromolecular stability. A given type of osmolyte generally has similar effects on DNA, RNA, prot...
Zhang Z, et al. antibiotic production in Streptomyces is organized by a division of labor through terminal genomic differentiation.Sci Adv. 2020; 6(3): eaay5781. Colizzi ER, et al. Evoltiion of genome fragility enables microbial divisions of labor.Mol Syst Biol. 2023; 19(3): e11353. ...
How does DNA replication differ in prokaryotes and eukaryotes? Where in the cell do transcription and translation occur in: a. prokaryotes? b. eukaryotes? How are rRNA genes organized in higher eukaryotes? How is the eukaryotic DNA packaged? Explain. ...
The biogenesis and processing of essential, independently transcribed snoRNAs is dependent on highly organized secondary structures and their sequential association with core snoRNP proteins that halt advancing exonucleases, and nucleolytic trimming of the precursor RNA is necessary to achieve functionality ...