Chobert JM, Tran V, Haertlé T (1998) How the substitution of K188 of trypsin binding site by aromatic amino acids can influence the processing of β-casein. Biochemical and biophysical research communications 246(3):847–858J.-M. Chobert, L. Briand, V. Tran, T. Haertle, How the ...
Proteins were digested overnight (550 rpm, 37 °C, 19 h) with 4 μg trypsin (1:50 protease to protein ratio) and digestion was terminated using formic acid (0.5% (v/v) final concentration). 2.7. Solid phase extraction (SPE) Pre-conditioned (1 mL methanol) and pre-equilibrated (1 ...
In all of these studies, there is always a fraction of soluble protein that does not attain a folded, functional state by the last time point. The percentage of molecules that did not fold ranged from a low of 6% to a high of 70% (Table1, Fig.1aand Supplementary Data1). Since struc...
"Avoiding toxins" does not solve the nutritional cost/benefit trade-off.On the other hand, as we shall see inPart 3,there is a trade-off between toxicity and deficiencies, in that if one tries to avoid as many toxins as absolutely possible, they would have to avoid so many foods that ...
What does the analysis of friction noise reveal about the mouthfeel of unfiltered protein solutions? How do the tested food proteins affect the salivary pellicle over time? What steps were involved in measuring the frictional response of the protein samples? What techniques were used to study the ...
This study investigated the differences in absorption of infant formula with different levels of α-lactalbumin (α-La) and β-casein (β-CN), and how α-La and β-CN (α+β) affect mineral absorption using the Caco-2 cell model. Compared to the control group, the free amino acid conte...
24 hr after transfection, cells were trypsinised and diluted into passaging medium containing 300 μg/ml G418 (Serva) in order to select for single clones harboring the transfected BAC. Positive clones were screen for by Western Blot and Live cell imaging. Cell lysis and immunoblotting HeLa ...