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Organs were homogenized in Trizol (Invitrogen) using Ultra-turrax T25 (IKA Labortechnik) immediately after collection and stored at −80 °C. The isolation of total RNA was performed according to manufacturer's instructions without DNase treatment. Total RNA dissolved in RNase free water, stored ...
Moreover, as sequencing depth increases, so too does the detection of cDNAs that likely correspond to spurious transcripts or genomic DNA contamination. Finally, even when dozens of barcoded individual cDNA libraries are sequenced in a single lane, the vast majority of transcripts in each sample ...
RNA was isolated from homogenized zebrafish embryos through the use of TRIzol (Thermo Fisher Scientific), and genomic DNA was removed using the TURBO DNA-free Kit (Thermo Fisher Scientific). Reverse transcriptase was performed using the ProtoScript II First Strand cDNA synthesis kit (New England Bio...
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RNA was isolated from homogenized zebrafish embryos through the use of TRIzol (Thermo Fisher Scientific), and genomic DNA was removed using the TURBO DNA-free Kit (Thermo Fisher Scientific). Reverse transcriptase was performed using the ProtoScript II First Strand cDNA synthesis kit (New England Bio...