especially to structural chemistry. To help the transfer of new knowledge in drug design practice, we reconsider a few vibrant topics of protein dynamics engaged in makingpredictionsbased on the timing of the events that are simulated.
Electrophysiological Measurements: Parameters such as short-circuit current (Isc) and transepithelial resistance (TER) are measured to investigate transport mechanisms and channel functionality, including the activity of the cystic fibrosis transmembrane conductance regulator (CFTR) in diseases like cystic fibro...
Cellular and Molecular Life Sciences (2020) 77:3085–3102 https://doi.org/10.1007/s00018-020-03473-3 REVIEW Cellular and Molecular Life Sciences Why and how to investigate the role of protein phosphorylation in ZIP and ZnT zinc transporter activity and regulation T. E. ...
For example, despite the fact that the CFTR gene was cloned some years ago, our understanding of precisely how it causes cystic fibrosis is still limited. The correlation between genotype and phenotype is also unclear for mutations in many other ion channel genes. It is often not possible to ...
Human airway epithelial (HAE) cellular models are widely used in applicative studies of the airway physiology and disease. In vitro expanded and differentiated primary HAE cells collected from patients seem to be an accurate model of human airway, offeri
This made it possible to distinguish between a wild type, a single point mutation (1653C/T), and a triple deletion (DeltaF 508) in the CFTR gene at the 0.02 aM level. It is worth mentioning that the majority of the capture DNAs used in SERS-based sensors are modified with a thiol ...
However, current clinical practice is lagging behind and does not yet target modifiable external factors that could alter disease progression. In this review, we will discuss cumulative and recent advances in ADPKD research that have led to the conclusion that the current concept of regarding ADPKD ...
Epidermal growth factor stimulates the disruption of gap junctional communication and connexin43 phosphorylation independent of 12-0-tetradecanoylphorbol 13-acetatesensitive protein kinase C: the possible involvement of mitogen-activated protein kinase. Modulation of Cx43 and gap junctional intercellular communic...
CF is caused by mutations in the CF gene. These mutations lead to defects in a specific protein called the cystic fibrosis transmembrane conductance regulator (CFTR) protein. As a result of these defects, the CFTR proteins don’t work the way they should. This is where TRIKAFTA comes in. ...
Over the last two decades, the understanding of how dysregulated ion channels and transporters are involved in carcinogenesis and tumor growth and progression, including invasiveness and metastasis, has been increasing exponentially. The present review s