qPCR can be divided into dye-based methods (e.g. SYBR® Green) and probe-based methods (e.g. TaqMan®). To learn more about these two types of qPCR assays, their validation and data analysis, read our blog post qPCR: How SYBR Green and TaqMan real-time PCR assays work....
Real time plasmonic qPCR: how fast is ultra-fast? 30 cycles in 54 secondsPolymerase Chain Reaction (PCR) is a critical tooldoi:10.1039/C7AN00304HRoche, Philip J. R.Najih, MohamedLee, Seung S.Beitel, Lenore K.Carnevale, Matthew L....
Ask TaqMan #34 Copy Number Variation – How Does It Work? microRNA Quantification using TaqMan® Advanced miRNA Assays requires a cDNA synthesis step followed by the detection using real-time PCR. The TaqMan Advanced miRNA Assay workflow. ...
A group at Symbolics is developing a Lisp runtime kernel, derived from it's Genera 1 operating system, to support real-time control applications. The first candidate application has strict response-time requirements (so strict that it does not permit the use of paged virtual memory). Traditional...
The sample is combined with both assays, master mix, and then run in quadruplicate in the real-time PCR instrument. Let’s look at an example in which the target gene is on the X-chromosome. In a male sample, we should then find a copy number of 1 for the target. ...
根据下列材料，作答106、107题。 甲与乙生意上有矛盾，遂指使身强体壮的丙找机会替自己教训乙。某日丙与女朋友逛街时同乙偶遇。丙借故对乙进行殴打，乙抓起路边商家铁锹进行回击。路人丁报警，派出所民警及时赶到现场。经鉴定，乙、丙都是轻微伤。 关于本案，下列说法正确的有（）。
The immune system is a complex network of specialized cells that work in concert to protect against invading pathogens and tissue damage. Imbalances in this network often result in excessive or absent immune responses leading to allergies, autoimmune dis
Avoiding cross-contamination during manual qpcr set-up is essential for reliable results, but can be a tedious, time-consuming process. Using an automated liquid handling system prevents cross-contamination and improves efficiency. Problem: Real-time qPCR enables amplification, detection, and qua...
The delta-delta Ct method, also known as the 2–∆∆Ctmethod, is a simple formula used in order to calculate the relative fold gene expression of samples when performing real-time polymerase chain reaction (also known as qPCR). The method was devised by Kenneth Livak and Thomas Schmittgen...
Promega storage buffers have been designed after accelerated and real time/real temperature stability experiments. All enzyme storage conditions are validated through our Quality Assurance re-assay program to maximize long term stability. Setting up digests with a single restriction enzyme is relatively ...