RT-PCR, not to be confused with real-time PCR, stands for reverse transcription PCR and can be used to amplify RNA target sequences. It involves an initial incubation of the sample RNA with a reverse transcript
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Explain what would happen if the PCR reaction did not contain Taq polymerase. Assuming all other required PCR components are present, would any part of the PCR reaction occur? Explain DNA amplification (PCR) and give one example of when it would be used. The polymerase chain reaction does not...
1.Reagent kit for Probe Labeling 2.Oligonucleotides for PCR amplification Whats one way in which electrophoresis is used in medicine today? Explain your findings. Why are the molecular cells in a compound useful in biomedical engineering? How can biotechnology help the environment? How is cell ...
TaqMan® Mutation Detection Assays are based on a novel competitive allele-specific TaqMan (castPCR™) technology, which combines allele-specific TaqMan qPCR with an allele-specific MGB blocker oligonucleotide to effectively suppress nonspecific amplification of the nontarg...
A pulsed current is fed to the heater (1); and a thermal cycle of PCR is carried out so that high-speed PCR amplification reaction can be achieved. The PCR reaction vessel (3) is placed within a heating block (5) which is maintained at a temperature close to the annealing temperature....
One workaround to help avoid nonspecific amplification is to prepare the PCR reaction mixture on ice. The colder temperature helps lower the activity of the DNA polymerase; however synthesis of undesirable products may still occ...
The amplification of DNA is achieved through a process called polymerase chain reaction (PCR). PCR works by repeatedly heating and cooling DNA. When DNA is heated, it separates into two single strands. Next, an enzyme called “Taq polymerase” is introduced to the sample. This enzyme ...
After adding the nucleic acid to be detected, the reaction system performs a fluorescent PCR amplification reaction. The time is slightly different depending on the PCR reagents and instruments used. Generally,it takes 90-120 mi...
Figure 1. Schematic and example of a DNA mismatch detection assay. Genomic DNA (blue) from edited cells contains wild type and edited DNA (mutation in red). PCR amplification around the editing site generates wild type and edited PCR products (black). Denaturing and reannealing of these produc...