Based on this strategy, the tool uses far more information than both TMM and DESeq strategies, which are usually restricted to a small subset of OTUs due to the a priori exclusion of null values. Pipelines The tools previously introduced were combined to form 35 different preprocessing pipe- ...
Another very well-known tool for RNA-sequencing analysis isDESeq2[39], which performs count data normalization and differential analysis. Also in this case, normalization is done through data scaling for sample-specific size factors. To estimate these size factors, DESeq2 package implements the me...