CRISPR RNA (crRNA) and Cas9:CRISPR DNA serves as a permanent record of past infections, but for bacteria to use these sequences to thwart viruses, they must convert them into DNA's cousin,RNA. Through a process called transcription, bacteria first copy one of the two CRISPR DNA strands int...
One of the RNAs, called CRISPR-related RNA (crRNA) encodes the target in its 5′ terminal, while the rest is partially complementary to a second RNA, the transactivator of crRNA (tracrRNA). The crRNA-tracrRNA complex is recognized by Cas9 and together they find the complementary m...
Figure 2. Designing crRNA for Cas12a. The correct sequence along with some common errors are shown. Detailed explanation of the simple instructions: For crRNAs to be used with A.s. Cas12a, identify locations in your target region with a PAM sequence, TTTV, where V is A, C, or G. Th...
. RdRp is the most sensitive and effective target for detecting SARS-CoV-2, and its tests have been validated in numerous laboratories (Caruana et al2020). Ishige et al. (2020) used three genes in the (rRT-PCR) assay for SARS-CoV-2 RNA detection: the SARS-CoV-2 specific N gene, t...