Samples were concentrated in a vacuum centrifuge. Partial Deglycosylation of O-Glycopeptides (14) Sialic acid and β-galactose units of glycopeptides were removed by incubation with neuraminidase (5–10 U/sample, New England Biolabs P0720; in 100 mm sodium citrate, pH 6.0) for 1 h at 37 ...
After incubation, the sample was centrifuged, and the supernatant was applied to a size exclusion chromatography (SEC) column (Hiload 26/600 Superdex 200 pg) to separate the monomers from the oligomers (Fig. 2B). Analysis of these fractions by SDS-PAGE under denaturing conditions showed the ...
Ticks are the vector of many human and animal diseases; and host detection is critical to this process. Ticks have a unique sensory structure located exclusively on the 1st pairs of legs; the fore-tarsal Haller’s organ, not found in any other animals, p
(Avanti J-E Centrifuge, Beckman Coulter, Brea, CA, USA) during 15 min, at 4 °C. The first centrifugation aimed at removing the supernatant, the plasma, and then three subsequent centrifugations were performed to wash the precipitate with sodium chloride (0.9%w/v, Fisher BioReagents, ...
International Journal of Molecular Sciences Article Tick Haller's Organ, a New Paradigm for Arthropod Olfaction: How Ticks Differ from Insects Ann L. Carr 1, Robert D. Mitchell III 1, Anirudh Dhammi 1, Brooke W. Bissinger 2, Daniel E. Sonenshine 3 and R. Michael Roe 1,* 1 Department ...