When calculating melting temperatures using online tools, such as the IDT SciTools™ OligoAnalyzer™ Tool and PrimerQuest™ Tool, be sure to enter the appropriate conditions from your PCR or qPCR experiment. Commonly used parameters are: 50 mM K+, 3 mM Mg2+, and 0.8 mM dNTPs; however...
Primer Design for PCR Primers are always specified 5′ to 3′, left to right. Verify that your primers are designed and ordered in the correct orientation. Primers for PCR and sequencing should be between 18 to 25 nucleotides in length. Primers for PCR and sequencing should have a GC co...
There is provided a method for designing primers for multiplex PCR, for assigning priorities to candidate amplification regions on same chromosomal DNA and designing primers for PCR amplifying the candidate amplification regions according to the priorities, the method having a feature in a method for...
How are primers involved in the replication of DNA? Describe DNA replication. DNA replication is a natural process whereas the Polymerase Chain Reaction is preformed within the lab to amplify small amounts of DNA. List the "ingredients" necessary for PCR and ...
Does PCR use RNA primers? What is DNA polymerase used for? How are a retrovirus and a lysogenic virus similar? What would happen if you only had one primer in a PCR reaction? How does a DNA virus reproduce its host genome? How does RNA participate in DNA replication? How can DNA sequ...
aim of this study is to test the impact of three experimental factors on the precision of the estimated PCR efficiency: (1) the effect if qPCR instrument changes; (2) the effect of how many technical replicates are included; and (3) the effect of the volume transferred across dilutions. ...
If your standard curve and primer efficiency is not within the desired range, don’t worry. There are a few things you can do to improve your PCR primers efficiencies, such as adjusting the primer concentrations and the annealing temperature of your reaction. If you are really struggling after...
This guide introduces restriction enzymes, providing in-depth reference information and tools to help you find buffers for double digests, or find enzymes by name or recognition sequence. Restriction Enzyme Tools are available for desktop or mobile....
researchers often resort to affordable Sanger sequencing methods. Preparing DNA samples for sequencing is an important process in itself, which involves primer design, extraction of genomic DNA, and PCR amplification. Download our detailed Genotyping Protocol below for more sample preparation and Sanger ...
Why are primers needed for DNA replication? What are primers made of in DNA replication? What is the role of DNA polymerase in DNA replication? Is DNA replication and transcription the same? What are primers in DNA replication? Define DNA replication ...