Genomic DNA or sorted cells are diluted to a single proviral endpoint and used for multiple displacement amplification (MDA). (Center) MDA reactions are screened for HIV-1 proviruses by amplification and sequencing of a sub-genomic region of HIV (e.g., LTR, gag, int, env), qPCR/ddPCR ...
Map-based cloning showed that the affected gene in the prc1 mutant is a CesA previously designated as AtCesA6[9]. Interestingly, the prc1 mutant phenotype is very similar to that caused by leaky alleles of rsw1, and a cross between prc1-8 and rsw1-10 resulted in a double mutant that...
Historically, antibiotics have been used to preventS. suiscases, but this practice is nowadays prohibited in many countries. Also, vaccination is used to prevent infection, but its efficacy is limited. Only bacterins are applied in the field for immunization of piglets or sows. Bacterins are ...
Spm, that was described as a disease model but never cloned (Miyawaki et al.1982) are both truncating mutations that appear to be complete null alleles with no detectable