PCRHot Start PCRPrimer dimerMisprimingThermal cyclerDNA polymerasedNTPsHot Start activation approaches are increasingly being used to improve the performance of PCR. Since the inception of Hot Start as a means of blocking DNA polymerase extension at lower temperatures, a number of approaches have been...
Hot-start PCR A common problem that results in spurious product formation is associated with the assembly of reactions at room temperature or on ice in a nonhot-start format: during the initial ramping (raising the reaction temperature) to 94°C needed to achieve initial melting and then to ...
14001014 Platinum™ II Hot-Start Green PCR Master Mix (2X) 1000 reactions 6,206.00 Online exclusive 初始Platinum Taq DNA 聚合酶 货号产品名称规格单价(CNY)数量 10966018 Platinum™ Taq DNA Polymerase 120 reactions 1,101.00 10966026 Platinum™ Taq DNA Polymerase 300 reactions 2,838.00 10966034...
www.toyobo.co.jp/e/bio F1788K Hot Start TTx (RNA) Kit HSTTX-111 250U Store at -20°C Contents [1] Introduction [2] Components [3] Protocol 1. Standard reaction setup 2. Cycling conditions [4] Example [5] Related products CAUTION All reagents in this kit are intended...
▪ 2X Phusion Green Hot Start II High-Fidelity PCR Master Mix (provides 1.5 mM MgCl2 in the final 1X concentration) ▪ Water, nuclease-free ▪ DMSO Store at -20°C. 相关产品: ▪DNA 片段标准品 ▪DNA ladder ▪DNA 聚合酶 ...
4.Placethereactionsinaroom-temperaturethermalcycler.PerformPCRusingyourpropertiesof.SeeBioMathCalculatortocalculatethemeltingtemperaturefor standardparameters.A2-minuteinitialdenaturationstepat94–95°CisprimersintheGoTaq®reaction(). requiredtoinactivatetheantibodyandinitiatehot-startPCR.•Minimizetheeffectofamplifi...
Product TypeHot Start High-Fidelity PCR Master Mix Shipping ConditionDry Ice For Use With (Application)Hot-start PCR, High-fidelity PCR Concentration2X Fidelity (vs. Taq)52X Hot StartBuilt-In Hot Start No. of Reactions100 Reactions OverhangBlunt Reaction FormatSuperMix or Master Mix Size (Final...
at standard cycling tmperatures. The new Affibody molecule utilized in the Phusion Hot Start II DNA Polymerase sets no temperature limitations in the annealing step of the PCR protocol. This improvement allows the use of a very wide range of primers, including those with low melting temperatures...
Protocol for Phusion®Hot Start Flex DNA Polymerase (M0535) 使用指南 Activity of Restriction Enzymes in PCR Buffers Guidelines for PCR Optimization with Thermophilic DNA Polymerases 工具& 资源 选择指南 DNA Polymerase Selection Chart Thermophilic DNA Polymerases ...
产品类型:PCR 及DNA聚合酶 品牌:Thermo Fisher 货号:EP1711 规格:200 U 价格:506 货号:EP1712 规格:500 U 价格:1126 货号:EP1713 规格:2500 U 价格:4749 货号:EP1714 规格:4 x 2500 U 价格:17351 产品介绍 技术参数 相关产品 产品介绍 描述