Hospital Chief Nursing Officer Carlsbad NM Hospital Vice President Finance Thedford NE Rural Hospital Registered Nurse Gothenburg NE Hospital Risk Management Coordinator Hermiston OR Hospital Pharmacist Oneonta NY Hospital Occupational Therapist Santa Clarita CA Hospital CFO Star ID Hospital Human Resources Di...
Research on the neonatal microbiome has been performed mostly on hospital-born infants, who often undergo multiple birth-related interventions. Both the hospital environment and interventions around the time of birth may affect the neonate microbiome. In
Committed to delivering quality health care to the communities of Artesia, Roswell, Carlsbad and Southeastern New Mexico.
Here we report the development of a mobile nucleic acid amplification testing (mobiNAAT) platform utilizing a mobile phone and droplet magnetofluidics to deliver NAAT in a portable and accessible format. By using magnetic particles as a mobile substrate for nucleic acid capture and transport, fluid...
, rabbit anti-GFP (Invitrogen, Carlsbad, Calif.), guinea pig anti-GFAP (Advanced Immunochemical, Long Beach, Calif.) and goat anti-GAD67 (Millipore, Billerica, Mass.). Tissues were incubated in primary antibody at 4° C. for 48-72 hours then washed three times with PBS. After washing,...
Each PCR reaction was assembled in agree- ment with 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA). Libraries were quantified by Qubit fluorometer (Invitrogen Co., Carlsbad, CA) and pooled to an equimolar amount of each index-tagged sample up to a 4 nM concen- ...
Finally, the bacteria were washed and suspended in 100 μl HBSS/BSA supplemented with 10 μg/ml of the DNA-binding fluorescent dye Hoechst 34580. The fluorescence intensity reflecting bound antibodies was read by an Attune flow cytometer (Life Technologies, Carlsbad, CA, USA), gating on the ...
extending the primers with PCR, and cloning them into pCR 2.1-TOPO vector using the TOPO TA Cloning Kit (Invitrogen Life Technologies, Carlsbad, Calif.). Head-to-head 21 bp shRNA fragments were PCR amplified using as a template the resulting tRNA-valine promoter in the Topo TA vector, the...
29.A method of detecting a very high density, ultra small, lipid-depleted particle in a biological sample, wherein the particle comprises apoB and a cytokeratin 8 polypeptide, and wherein the particle has an average particle diameter in a range of from about 7.1 nm to about 22 nm, has ...