Genotyping for GWS currently uses an 8K SNP array, but is likely to transition to ‘genotype-by-sequencing’ in the near future. GEBVs are calculated from genotypic data exclusively. The application of GWS relies on the use of a training population for development of the model for association...
This method results in a value for the maximum possible breakdown rate of the rooting material tested and allows ranking of materials in classes for stability over time. Media were mixed with a nutrient solution (4.3 g·L−1 NH4Cl, 5.4 g·L−1 CaCl2·2H2O, 4.3 g·L−1 MgSO4·7...
There are several types and classes of CRISPR/Cas systems [10–12]. The most convenient and often used system for genome editing is the CRISPR/Cas system with an effector nuclease, Cas9 (class II and type II) from the bacterium Streptococcus pyogenes [13]. Recently, other, more advanced ...