The RT-PCR protocol was carried out manually. cDNA was synthesized in a total volume of 20 μL/well in the above 96-well plate which included 2 μL (50 U) Maxima H minus reverse transcriptase (Thermo Scientific) in DEPC-treated water into each well. The total RNA of each B cell (de...
The present invention provides nucleic acid aptamers that bind to Plasmodium proteins lactate dehydrogenase and histidine-rich protein II, and uses thereof for the diagnosis of malaria. Aptamers again
Gly has been identified as the major FAA of Hong Kong oyster that functions as osmolyte in response to osmotic stress, and genes related to intracellular Gly were supposed to be essential for osmotic regulation. In this study, three genes, CHSHMT1, CHGLDC and CHALAS, were investigated, and ...
the patient's tumor harbored MAPK1p.E322K mutation, which was then subsequently proven to confer heightened sensitivity to erlotinib in vivo6,7. Here, 105 HNSCC tumors from Hong Kong were sequenced, and two other MAPK1 mutations: p.R135K and p.D321N, were identified in recurrent...