Fruit FliesDrosophila MelanogasterFly TransferImmobilization TechniquesHomemade ToolsMicropipette TipsMicrocentrifuge TubesEgg Collection CaseInsect PinsMechanical PencilsFunnel StopperVial Transfer Play Video PDF DOI DOWNLOAD MATERIALS LIST Cite This Article Yang, D. Simple Homemade Tools to Handle Fruit...
2.3. SLiCE reaction for seamless DNA cloning SLiCE buffer (10×; 500 mM Tris–HCl, pH 7.5, 100 mM MgCl2, 10 mM ATP, and 10 mM dithiothreitol) was passed through a 0.2-μm filter, and dispensed in 40 μL aliquots into 0.2 mL 8-strip PCR tubes and stored at −20 °C [6]. Th...
Then guests can choose from an assortment of test tubes filled with edible eyes, sprinkles, dirt (made from crushed OREO cookies), etc., to create their science experiment. A little of this, a little of that, then slowly add the Orange Fanta and watch everything bubble and foam up just...
For convenience, we describe below the method for the extraction of RNA from 100 to 200 mg of tissue using 1.5-ml microcentrifuge tubes. Starting with 200 mg will yield about 5 μg/μl total RNA. Depending on the required amount of RNA, scale appropriately. Cell lysis and protein ...
I used Tom’s of Maine toothpaste for years because of its natural ingredients but also (mostly) because of its metal rather than plastic (plastic = bad) tubes. Then, mysteriously, I one day went to buy toothpaste and all the Tom’s of Maine tubes had switched to plastic, just like al...
The dry mass was discarded, while the solution was distributed in 50 mL centrifuge tubes and centrifuged at 5000 rpm for 10 min in a bench-top centrifuge. The supernatant was collected and stored at −20 ◦C. The extract contained ~22% ethanol (measured using Megazyme enzymatic ethanol ...