(RT-PCR) cloning of mRNA from various rat tissues (Schworeret al., 1993;Mayeret al., 1993).There are interesting similarities between these and the other reported subunit splice variants, indicating some conservation of structure in the variable region. For example, α-subunits and the δ2-...
DNA polymerase III holoenzyme contains two DNA polymerases embedded in a particle with 9 other subunits. This multisubunit DNA polymerase is the Eschericia coli chromosomal replicase, and it has several special features that distinguish it as a replicating machine. For example, on...
For example, while α interacts with the β2 clamp (KD = 800 nM), the Pol III core (αɛθ) does so more strongly (KD = 250 nM); this was proposed at the time to be a consequence of ɛθ contributing to the conformational stabilization of α [225]. As discussed below,...
For example, the Orc1/Cdc6 initiator proteins, the MCM helicase, processivity clamp PCNA, DNA primase and RFC clamp loader are near ubiquitous2,3. However, the DNA polymerases of archaea show a surprising level of diversity5. DNA polymerases can be classified into one of six families, A, ...
The study was performed using biolayer interferometry (BLI) with GST peptides immobilized on anti-GST sensors and titrated concentrations of B′ subunits and holoenzymes in the mobile phase (Figure 2b; example spectra for GST-DENND2C are shown, and results for all peptides are summarized in ...
The study was performed using biolayer interferometry (BLI) with GST peptides immobilized on anti-GST sensors and titrated concentrations of B′ subunits and holoenzymes in the mobile phase (Figure 2b; example spectra for GST-DENND2C are shown, and results for all peptides are summarized in ...
aSetting an efficiency goal provides us with a power loss budget. For example, if we want 90% efficiency,we can calculate what the 10% loss would be (our power loss budget) and design the sum of the power loss for all components to be no greater than that bud- 制定效率目标提供我们以...
example, NtrC (nitrogen regulatory protein C, also designated as NR I ) that are mediated by DNA- looping (Collado-Vides et al., 1991; North et al., E-mail address of the corresponding author: Karsten.Rippe@dkfz-heidelberg.de Abbreviations used: SFM, scanning force microscopy; ...
The IVβ mutant thus behaves similarly to the Rubisco SSU antisense plants analysed by Stitt and co‐workers (summarised by Stitt and Schulze 1994) and gives an example of the possible influence of plastome mutations on the sensitivity of the photosynthetic apparatus to excess light by modifying ...
An example of a highly regulated sigma 70 promoter is the ribosomal promoter, which surprisingly, appears to be regulated by nucleotide rather than macromolecule effectors.; The focus of this dissertation is to better understand the roles of nucleotides in regulation of these sigma RNA polymerases. ...