限公司 (货号 TSK301M,TSE201);细胞凋 亡试 剂盒购自杭州联科生物技术有限公司 (货号 AP104);Bax,Bcl-2 抗体购自 Abmark 公司 (货号 TU333334S,TU323153S),PARP-1,α-actin抗 体购自 proteintech 公司 (货号 23660-1-AP,66520-1-Ig),HO- 1,Nrf2 抗体购自 ABclonal 公司 (货号 A1244, A1346)....
minutes each time and incubated with goat anti-rabbit immunoglobulin G (IgG) (H + L) horseradish peroxidase (HRP) (1 : 1,000 dilution, AS014; ABclonal) or goat anti-mouse IgG (H + L) HRP (1 : 5,000 dilution, AS003; ABclonal) at room temperature for 2 hours. ...
antibody (1:500, Invitrogen, CA, USA), rabbit anti-Nrf2 polyclonal antibody (1:1000, Proteintech, Wuhan, China), rabbit anti-HO-1 polyclonal antibody (1:1000, Proteintech), rabbit anti-NLRP3 antibody (1:1000, Abclonal, Wuhan, China), rabbit anti-caspase-1 antibody (1:1000, Abclonal),...
, P53 (1: 1000; A0263, ABclonal Technology, Shanghai, China), P21 (1: 1000; A1483, ABclonal Technology, Shanghai, China), P16 (1: 1000; 10883-1-AP, Proteintech Group, Inc., Wuhan, China), Hdac2 (1: 1000; A2084, ABclonal Technology, Shanghai, China) or β-actin (1: 20000;...
Anti-HO-1 was purchased from ABclonal Inc. (College Park, MD, USA). Anti-NF-κB p65 was from Beyotime (Shanghai, China). ECL Western blotting detection kit, horseradish peroxidase (HRP)-conjugated anti-rabbit immunoglobulin G (IgG), and anti-mouse IgG were purchased from Huaxingbio. (...
Anti-NADPH quinone oxidoreductase-1 (NQO1, #A19586), anti-superoxide dismutase-2 (SOD2, #A1340), anti-B cell lymphoma 2 (BCL-2, #A20777), and anti-BCL-2-associated X protein (BAX, #A11931) were purchased from ABclonal Technology (Wuhan, China). Anti-α-Tubulin antibody and HRP ...
(1:1000, ABclonal, A1253),ACSL4(1:500, ABclonal, A16848),GPX4(1:1000, Abcam, ab125066),FTH1(1:500, ABclonal, A19544), HO-1 (1:500, ABclonal, A1346) Nrf2 (1:500, ABclonal, A0674), and actin (1:5,000, ABclonal, AC026) 4 °C overnight. Secondaryantibodies conjugatedwith ...
After being washed with PBS for two times, the cells were incubated with rabbit ALP anti- bodies (A0514, 1:200, Abclonal, China) diluted in 1% BSA overnight at 4℃. The cells were washed in PBS and again incubated with secondary antibodies, AlexaFluor 488 goat anti-rabbit IgG (H+L)...