hnRNP proteins and splicing control. Martinez-Contreras Rebeca,Cloutier Philippe,Shkreta Lulzim,Fisette Jean-Fran?ois,Revil Timothée,Chabot Benoit. Advances in experimental medicine and biology . 2008Martinez-Contreras, R., Cloutier, P., Shkreta, L., Fisette, J.F., Revil, T., and Chabot, B...
Understanding how RNA binding proteins control the splicing code is fundamental to human biology and disease. Here, we present a comprehensive study to elucidate how heterogeneous nuclear ribonucleoparticle (hnRNP) proteins, among the most abundant RNA binding proteins, coordinate to regulate alternative...
Recent progress in the study of alternative RNA splicing indicates that the interaction of RNA-binding proteins with specific target elements modulates splice site recognition and spliceosome assembly. The identity of splicing signals, the presence of modulating elements and differences in the distribution...
One of the proteins that was significantly differentially phosphorylated in each of these datasets was hnRNP M. hnRNP M is a splicing factor and RBP that has been repeatedly implicated in cancer metastasis (Chen et al., 2014, Passacantilli et al., 2017, Thomas et al., 2011, Xu et al.,...
Heterogeneous nuclear ribonucleoproteins (HNRNPs) represent a large family of RNA-binding proteins consisting of more than 20 members and have attracted great attention with their distinctive roles in cancer progression by regulating RNA splicing, transc
Mutant exon 6D sequences function as a splicing enhancer when inserted into an enhancer-dependent splicing construct. hnRNP H family proteins bind to the enhancer as well; their binding is dependent on the sequence GGGA located just downstream of the point mutation and depletion-- reconstitution ...
Mass spectrometry analysis of the excised bands disclosed that the molecule bound to the wild-type RNA probe was SRSF1, and that to the p.E415G RNA probe was hnRNP H. We confirmed the identity of bound proteins by immunoblotting with specific antibodies against SRSF1 (32–4500, Invitrogen)...
The molecular basis of cell signal-regulated alternative splicing at the 3' splice site remains largely unknown. We isolated a protein kinase A-responsive ribonucleic acid (RNA) element from a 3' splice site of the synaptosomal-associated protein 25 (Snap25) gene for forskolin-inhibited splicing ...
General and Specific Functions of Exonic Splicing Silencers in Splicing Control 5' splice sites, and tethering of hnRNP A1 and SF2/ASF proteins between competing splice sites mimicked the effects of ESS and ESE elements, ... Z Wang,X Xiao,EV Nostrand,... - 《Molecular Cell》 被引量: 336...
antibodies raised against synthetic hnRNP-M peptides can directly inhibit in vitro splicing, Furthermore, stressing cells at 45 degrees C for 10 min is sufficient to abolish the splicing capacity of subsequently prepared nuclear extracts which, interestingly, do not contain the hnRNP-M proteins any...