This method especially in the HLA typing which is based on DNA, in addition, we have conformed to combining with the appropriate selection of the region which is tested, former HLA typing method compared to operation is simple. We identified the group of these analyses concerning HLA-A, HLA-...
Comparison of Two HLA Typing Methods Yuan Yingbo1,Fang Ming2 (1.Heilongjiang Bayi Agricultural UniversKy,Heilongjiang Daqing163319 ,China；2.JiMei UniversKy,Fujian Xiamen361021,China)Abstract:Comparing the accuracy of two HLA typing methods,we typed the whole genome exon sequencing data(WES)of 100...
Several molecular techniques have been adapted for the human leukocyte antigen (HLA) typing with their advantages and drawbacks, which greatly improved the feasibility and precision of HLA typing as compared to previously used methods. Molecular techniques enabled more accurate analyses of the influence ...
Methods Mol Biol,2006,333(8):157-174. [6] Gabriel C,Fürst D,Faé I,et al. HLA typing by next-generation sequencing - getting closer to reality[J]. Tissue Antigens,2014, 83(2):65-75. [7] Schöfl G, Lang K, Quenzel P,et al. 2.7 million samples genotyped for HLA by next ...
typing.In allthese HLADAN typingmethods,PCRsequencing—basedtyping(SBT)is themostaccurateandreliablemethod.Our objective inthe proj ectisestablisheda complete,accurateandreliable HLA—BallelePCR—SBTmethod,using DNA sequencing, single—chainspecificprimerssequencing andclonetechnique. MethodsOursamplescomefromra...
The HLA high-resolution typing test was performed using polymerase chain reaction (PCR) amplification and sequencing-based typing methods. Genomic DNA was extracted from [Patient's Sample], and the HLA loci (A, B, C, DRB1, DRB3/4/5, DQB1, DPA1, and DPB1) were analyzed using a commerc...
热度: HLA 配型的原理与技术目录 热度: HLA配型技术 临床输血与检验2006年1O月第8卷第4期 HLA配型技术 姚萍王保龙沈建军 ?347? 【摘要】人类白细胞抗(HLA)是人类最复杂的遗传系统,其高度的多 态性影响着细胞和器官移植的免疫反应,并与一 些感染性疾病的进程有关,因此,准确的HLA分型技术无疑有着重要 ...
The present invention relates to methods and materials for determining the HLA Class I type of a subject, wherein group-specific sequences are used to design primer molecules which may be used in amplification protocols which accurately identify the HLA group(s) and/or allele(s) carried by ...
MethodsTwelve renal function failure patients with population reactive antibody (PRA) positive rate of 36% to 76% accepted successful HLA matchi 4、ng by using specific monoclonal antibodies HLA-, HLA- antigen typing dishes. ResultsThree recipients were in conformation with donors in one antigen at...
The maximal-relevance-minimal-redundancy (mRMR) and incremental feature selection (IFS) methods were then performed, based on the protein features, and a set of core factors that may affect the affinity of a biogenic or artificial peptide to certain HLA I molecules were discovered. A ...