Various lysates were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading contr...
人类白细胞抗原A抗体HLAClass1ABC/HLAABC免疫标记,Western Blot抗体杂交: 孵育一抗 A. 先将需要检测的抗体准备好,并决定好它们的稀释度。 B. 配好5%的Milk(TBST溶解),按要求稀释好抗体。注意,如需高比例稀释, 采用梯度稀释。 C. 将稀释好的抗体和膜一起孵育。 一般采用RT 1小时,可根据抗体量和膜上 ...
Various lysates were subjected to SDS PAGE followed by western blot with 15240-1-AP (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.View All Images (11)HLA class I ABC Polyclonal antibody HLA class I ABC Polyclonal Antibody for WB, IHC, IF/...
Low-molecular-weight protein western blot was performed using a 12% Bis-Tris gel (ThermoFisher) under denaturing conditions with an SDS running buffer70. Protein transfer to a PVDF membrane was performed using the iBlot 2 system (ThermoFisher) at 20 V for 4 min. Following the transfer,...
F Western blot analysis for HLA-E and GAPDH in a panel of MM cell lines (U266, RPMI-8226, OPM-2, and H929) treated with solvent, IFN-γ 1 ng/mL, or IFN-γ 50 ng/mL for 24 h. Full size image HLA-E expression correlates with CD56 and CREB1 expression in patients...
While normal levels of LMP2, LMP7, TAP1, TAP2 and HLA class I alleles on NPC tumour cells have been described in one study (Khanna et al, 1998), others have shown heterogeneous expression with a partial loss of HLA-ABC in almost 50% of NPC cases (Lai et al, 1990). A genome-...
Although HLA class II could be demonstrated intracellularly using Western blot on whole-cell lysates, it was not expressed on the cell surface. In coculture experiments, MSC failed to elicit proliferation of allogeneic lymphocytes. When undifferentiated MSC were exposed to IFN-γ, more than 90% ...
Purified complexes heavier than 100 kDa were analysed by western blot after reduction (Fig. 7A). As a negative control, identical amounts of proteins from HeLa cells were analysed following the same procedure. To exclude any excessive cross-linking of membrane proteins due to DTSSP treatment...
In malignant mesothelioma and breast carcinoma, HLA-G is only focally expressed, while HLA-ABC expression is not down regulated (Kleinberg et al., 2006). Up-regulated expression of HLA-G in malignant mesothelioma effusions and its association with shorter disease-free survival time in advanced st...
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