Currently, it is not clear how H9c2 and HL-1 cells mimic the responses of primary cardiomyocytes to hypoxia and oxidative stress. In the present study, we show that H9c2 cells are more similar to primary cardiomyocytes than HL-1 cells with regard to energy metabolism patterns, such as ...
基于线粒体生物发生探讨人参总次苷对缺氧下H9c2细胞凋亡和能量代谢的影响 热度: 人工智能基础(第2版) x2d;高济 x2d;ai x2d;4 x2d;本 热度: 线粒体泛素连接酶1对大鼠心肌细胞H9C2缺氧损伤的实验研究 热度: 相关推荐 H9c2andHL-1cellsdemonstratedistinctfeaturesofenergy metabolism,mitochondrialfunctionand...
细胞H9C2和小鼠心房肌细胞HL-1研究Apelin-13对Pannexin-1的蛋白水平,Pannexin-1半通道的开放,FAM134B的水平,内质网自噬和心肌细胞肥大的作用,在前期基础上进一步验证Apelin-13能否通过影响Pannexin-1蛋白的表达,并激活Pannexin-1半通道从而诱导FAM134B依赖性内质网自噬,作用于大鼠心肌细胞H9C2和小鼠心房肌细胞HL-1致使...
方法:1.RNA干扰:设计干扰链SiRNA-FAM134B抑制大鼠心肌细胞H9C2和小鼠心房肌细胞HL-1中FAM134B的表达;2.免疫荧光技术观察Apelin-13,APJ受体拮抗剂F13A,Pannexin-1半通道抑制剂Carbrnoxolone和Probenecid处理后大鼠心肌细胞H9C2和小鼠心房肌细胞HL-1中Pannexin-1和FAM134B的表达,以及内质网常驻蛋白KDEL与自噬标志蛋白...