Active infection can also be confirmed by enzyme-linked immunosorbent assays (ELISA) and immunofluorescence. Cryptococcus neoformans A Cryptococcus neoformans infection may manifest as an acute presentation of meningitis or meningoencephalitis. The most common symptoms of these disease states include fever,...
The neutralizing epitopes for these mAbs were specified by NS-EM and competition ELISA. Five NAbs targeted the gp41 subunit of the Env trimer, of which CM05G was imaged as a representative in NS-EM, confirming an epitope in gp41 (Fig. 4e). The non-V1V2V3 NAb CM04F neutralized the ...
Next, competitive binding of Fabs 66, 69 and 177 to gp41 was evaluated by competitive ELISA. Interaction of Fab 177 with gp41-MPR was completely inhibited by a 20-fold excess of Fab 69 (Figure 4c) and vice versa (data not shown). As these two Fabs have identical heavy chains but ...
2A and C). For both proteins, the IgG binding response reflected the trends observed in the ELISA assays of higer average binding for CTLA-4 and CTLA-4 plus PD-1 Ab-treated animals and lower average binding of PD-1 Ab-treated macaques (Fig. 2a and Supplementary Fig. 2C). There were...
the release of infectious virus was examined by HIV-1 p24 detection ELISA kit following the manufacturer’s instructions (Abcam, Cambridge, UK), and the absorbance was measured at a wavelength of 450 nm using an ELISA plate reader (BioRad, Hercules, CA, USA). The 50% inhibition concentration...
the release of infectious virus was examined by HIV-1 p24 detection ELISA kit following the manufacturer’s instructions (Abcam, Cambridge, UK), and the absorbance was measured at a wavelength of 450 nm using an ELISA plate reader (BioRad, Hercules, CA, USA). The 50% inhibition concentration...
To monitor any recruitment of the innate immune system by the siRNAs, TNFα, and interferon α production was measured by ELISA 24 h after either nucleofection of PHA-stimulated PBMCs (C) or transfection of RAW-ELAM cells (D). Cells were also treated with the control compounds ...
A fast non-radioactive ELISA protocol can be used to detect gag protein (DUPONT or COULTER gag antigen capture assay). [0061] At least three types of lentiviral vectors based on the gag/pol genes of the invention for use in gene therapy and/or as a vaccine are envisioned, i.e., ...
ELISA Assays Half-area 96-well ELISA plates were coated overnight at 4C with 50μL PBS containing 250 ng of compound per well. The wells were washed four times with PBS containing 0.05% Tween 20 and blocked with 3% BSA at room temperature for 1 h. Serial dilutions of sera were then ...
An ELISA test was used as a tiebreaker if there was disagreement in the results from the two rapid tests. Plasma samples from HIV-negative participants were tested for HIV using the Coulter p24 antigen assay and those found to be HIV negative were included as HIV ...